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人间充质干细胞脂肪生成qPCR检测试剂盒

英文名:Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit

货号:GK129

价格:$250

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ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit (GQH-MAD) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) adipogenic lineage commitment and differentiation. MSCs have the capacity to self-renew while maintaining stemness, and have the potential to differentiate into specialized cell lineages including adipocytes. The PPAR-gamma signaling pathway plays a key role in the induction and regulation of the adipogenesis process. Brief examples of how genes may be grouped are shown below:

  • MSC markers: ALCAM, ANPEP, CASP3, CD44, ENG, ERBB2, ITGA1/6/V, MCAM, NGFR, NT5E, PDGFRA/B, THY1, VCAM1, VIM
  • Stemness markers: FGF2, FUT4, INS, LIF, POU5F1, PROM1, SOX2, TERT, WNT3A, ZFP42
  • Adipogenic commitment regulation: ADIPOR1/2, AXIN1, CCND1, CEBPB/D, CIDEA, DLK1/2, EGR2, FGF1, FOXO1, GATA2/3, KLFs, LEP, LEPR, RHOA, SLC27A1, TNFRSF9, VDR
  • PPAR-gamma signaling: ADIPOQ, AGT, CEBPA, CREB1, FABP4, IRS2, KLF15, PPARG, RETN, SIRT3, SREBF1, STAT5A
  • Fully differentiated adipocyte markers: FASN, LIPE, LPL, PPARGC1A, SLC2A4, UCP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Plate Layout*

(8 controls in Bold and Italic)

1

2

3

4

5

6

7

8

9

10

11

12

A

ADIPOQ

AXIN1

CEBPB

DLK2

FGF2

INS

KLF5

LPL

PPARG

SLC27A1

TNFRSF9

ACTB

B

ADIPOR1

BMPR2

CEBPD

E2F1

FOXO1

IRS2

KLF6

MCAM

PPARGC1A

SLC2A4

UCP1

GAPDH

C

ADIPOR2

BSCL2

CFD

EGR2

FUT4

ITGA1

KLF9

NGFR

PROM1

SOX2

VCAM1

LDHA

D

ADRB2

CASP3

CIDEA

ENG

FZD9

ITGA6

LEP

NT5E

RETN

SREBF1

VDR

NONO

E

AGT

CCND1

CLTCL1

ERBB2

GATA2

ITGAV

LEPR

PDGFRA

RHOA

STAT5A

VIM

PPIH

F

ALCAM

CD44

CREB1

FABP4

GATA3

ITGB1

LIF

PDGFRB

SFRP1

TERT

WNT3A

GDC

G

ANPEP

CDKN1B

DKK1

FASN

GNL3

KLF15

LIPE

PLIN2

SFRP5

TFRC

ZFP42

PPC

H

ARNTL

CEBPA

DLK1

FGF1

HES1

KLF2

LMNA

POU5F1

货号GK129
产地美国
缩写GQH-MAD
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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