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人类先天淋巴细胞发育标记qPCR检测试剂盒

英文名:Human Innate Lymphoid Cell Development Markers qPCR Array Kit

货号:GK118

价格:$250

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ScienCell's GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Kit (GQH-ILC) is designed to facilitate gene expression profiling of 24 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into innate lymphoid cells (ILCs), natural killer (NK) cells, and lymphoid tissue inducer (LTi) cells. Brief examples of how included genes may be grouped according to their functions are shown below:

  • CLP markers: MME, CD34, IL7R, FLT3
  • Immature NK cell markers: CD33, PROM1, CD34, KIT, IL2RB, KLRK1
  • Mature NK cell markers: FCGR3A, IL2RA, NCAM1, B3GAT1, KLRD1, KLRC1, NCR1/2/3
  • ILC progenitor markers: CD34, KIT, IL7R
  • ILC markers: NCAM1, KLRD1, ITGAE, IL7R, KLRB1, PTPRC, NCR1, RORC
  • LTi cell markers: CD4, IL7R, CCR6

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

B3GAT1

IL2RB

MME

ACTB

B3GAT1

IL2RB

MME

ACTB

B3GAT1

IL2RB

MME

ACTB

B

CCR6

IL7R

NCAM1

GAPDH

CCR6

IL7R

NCAM1

GAPDH

CCR6

IL7R

NCAM1

GAPDH

C

CD33

ITGAE

NCR1

LDHA

CD33

ITGAE

NCR1

LDHA

CD33

ITGAE

NCR1

LDHA

D

CD34

KIT

NCR2

NONO

CD34

KIT

NCR2

NONO

CD34

KIT

NCR2

NONO

E

CD4

KLRB1

NCR3

PPIH

CD4

KLRB1

NCR3

PPIH

CD4

KLRB1

NCR3

PPIH

F

FCGR3A

KLRC1

PROM1

GDC

FCGR3A

KLRC1

PROM1

GDC

FCGR3A

KLRC1

PROM1

GDC

G

FLT3

KLRD1

PTPRC

PPC

FLT3

KLRD1

PTPRC

PPC

FLT3

KLRD1

PTPRC

PPC

H

IL2RA

KLRK1

RORC

NTC

IL2RA

KLRK1

RORC

NTC

IL2RA

KLRK1

RORC

NTC

 

* gene selection may be updated based on new research and development

货号GK118
产地美国
缩写GQH-ILC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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