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人类炎症趋化因子,白细胞介素和受体qPCR检测试剂盒

英文名:Human Inflammatory Chemokines, Interleukins, and Receptors qPCR Array Kit

货号:GK110

价格:$250

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ScienCell's GeneQuery™ Human Inflammatory Chemokines, Interleukins, and Receptors qPCR Array Kit (GQH-CHE) surveys 88 key genes involved in first steps of inflammation signaling.  Inflammation occurs as a protective response against harmful stimuli and as a signal to initiate tissue repair.  The initiation of inflammation involves immune cell migration to the insulted site by chemotaxis and immune cell activation by cytokines.  This kit focuses on chemokines and interleukins involved in the initiation of inflammation.  Note: For a survey of 88 additional cytokines involved in the initial inflammatory response, a separate GeneQuery array is available (GK109; Human Inflammatory Cytokines and Receptors).  Brief examples of how included genes may be categorized are shown below:

  • Chemokines: CCL1, CCL20, CCL5, CX3CL1, CXCL9
  • Chemokine receptors: CCR1, CCR5, CCR9, CX3CR1, CXCL2
  • Interleukins: IL10, IL2, IL6, IL12A, IL9
  • Interleukin receptors: IL10RA, IL2RA, IL6R, IL12RB1, IL9R

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Inflammatory Chemokines, Interleukins, and Receptors qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CCL1

CCL20

CCR1

CCR9

CXCL2

CXCR5

IL12RB1

IL17A

IL1R1

IL2RB

IL5

β-actin

B

CCL11

CCL22

CCR2

CCRL2

CXCL3

CXCR6

IL12RB2

IL17B

IL1R2

IL2RG

IL5RA

GAPDH

C

CCL14

CCL24

CCR3

CX3CL1

CXCL8

IL10

IL13

IL17C

IL2

IL3

IL6

LDHA

D

CCL15

CCL27

CCR4

CX3CR1

CXCL9

IL10RA

IL13RA1

IL17RA

IL20

IL31RA

IL6R

NONO

E

CCL17

CCL3

CCR5

CXCL1

CXCR1

IL10RB

IL13RA2

IL18

IL21R

IL32

IL7

PPIH

F

CCL18

CCL4

CCR6

CXCL10

CXCR2

IL11

IL15

IL18R1

IL23A

IL3RA

IL7R

GDC

G

CCL19

CCL5

CCR7

CXCL11

CXCR3

IL12A

IL15RA

IL1A

IL27RA

IL4

IL9

PPC

H

CCL2

CCL8

CCR8

CXCL12

CXCR4

IL12B

IL16

IL1B

IL2RA

IL4R

IL9R

NTC

 

 * gene selection may be updated based on new research and development

货号GK110
产地美国
缩写GQH-CHE
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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