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人类炎症细胞因子和受体qPCR检测试剂

英文名:Human Inflammatory Cytokines and Receptors qPCR Array Kit

货号:GK109

价格:$250

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ScienCell's GeneQuery™ Human Inflammatory Chemokines, Interleukins, and Receptors qPCR Array Kit (GQH-CHE) surveys 88 key genes involved in the first steps of inflammation signaling.  Inflammation occurs as a protective response against harmful stimuli, as a signal to initiate tissue repair, and involves the local vascular system.  Cytokines are an important facet of the initial inflammatory response and include chemokines, interleukins, lymphokines, and tumor necrosis factors.  This kit focuses on tumor necrosis factors (TNFs), interferons, and the initiation of a local vascular response.  Note: For a survey of 88 additional cytokines involved in the initial inflammatory response, a separate GeneQuery array is available (GK110; Human Inflammatory Chemokines, Interleukins, and Receptors).  Brief examples of how included genes may be categorized are shown below:

  • Interferon signaling: IFNA1, IFNA21, IFNAR1, IFNB1, IFNG
  • TNF signaling: TNF, TNFRSF10A, TNFRSF14, TNFSF10, TNFSF8
  • Vascular response signaling: GDF2, GPI, MDK, TYMP, VEGFA
  • Growth factors: FGF1, FGF2, GDF3, PDGFB, VEGFD
  • Other cytokines: CSF1, EPO, LTA, NAMPT, SPP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Inflammatory Cytokines and Receptors qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

BMP2

CSF3

FGF7

IFNA1

IFNA4

IFNG

MDK

PTN

TNFRSF11B

TNFRSF1A

TNFSF13

β-actin

B

BMP7

CSF3R

GDF2

IFNA10

IFNA5

IFNGR1

MIF

SPP1

TNFRSF12A

TNFRSF1B

TNFSF13B

GAPDH

C

CD40LG

EPO

GDF3

IFNA13

IFNA6

INHBA

MPL

TNF

TNFRSF13B

TNFRSF21

TNFSF14

LDHA

D

CD70

F3

GFRA1

IFNA14

IFNA7

INHBE

NAMPT

TNFRSF10A

TNFRSF13C

TNFRSF4

TNFSF4

NONO

E

CSF1

FASLG

GFRA2

IFNA16

IFNA8

LEPR

NODAL

TNFRSF10B

TNFRSF14

TNFRSF8

TNFSF8

PPIH

F

CSF2

FGF1

GHR

IFNA17

IFNAR1

LIF

PDGFA

TNFRSF10C

TNFRSF17

TNFRSF9

TYMP

GDC

G

CSF2RA

FGF10

GPI

IFNA2

IFNAR2

LIFR

PDGFB

TNFRSF10D

TNFRSF18

TNFSF10

VEGFA

PPC

H

CSF2RB

FGF2

GRN

IFNA21

IFNB1

LTA

PRL

TNFRSF11A

TNFRSF19

TNFSF11

VEGFD

NTC

 

 * gene selection may be updated based on new research and development

货号GK109
产地美国
缩写GQH-IFN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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