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人类ECM降解qPCR检测试剂盒

英文名:Human ECM Degradation qPCR Array Kit

货号:GK105

价格:$250

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ScienCell's GeneQuery™ Human ECM Degradation qPCR Array Kit (GQH-ECM) profiles 88 key genes involved in extracellular matrix (ECM) degradation.  The ECM comprises molecules secreted by cells that provide structural and biochemical support.  Its roles include supporting intercellular communication, growth factor storage, and regulating cell migration and motility.  Degradation of the ECM thus influences growth factor release, chemotaxis, cell migration, and cellular adhesion.  Enzymes classically involved in ECM degradation include serine proteases, threonine proteases, and matrix metalloproteinases.  Brief examples of how included genes may be categorized are shown below:

  • ECM components: ACAN, COL18A1, COL4A1, FGG, LAMA5, NTN4
  • Proteases: ADAM10, ADAMTS1, BMP2, CAPN9, CTSG, MMP2, MMP9
  • Protease inhibitors: A2M, CAST, SERPINH1, TIMP1, TIMP2
  • ECM proteoglycans: BGN, DCN, LTBP2, LUM, PCOLCE
  • Cell adhesion receptors and glycoproteins: CD44, CEACAM8, FN1, HSPG2, SPARC

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

 

GeneQuery™ Human ECM Degradation qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

A2M

ADAMTS4

CAPN1

CEACAM8

COL4A1

CTSG

FGB

LAMA2

LOX

MMP3

PLG

β-actin

B

ACAN

ADAMTS5

CAPN10

COL11A1

COL4A3

CTSS

FGG

LAMA3

LTBP2

MMP7

PSEN1

GAPDH

C

ADAM10

BGN

CAPN2

COL14A1

COL4A5

CTSV

FN1

LAMA5

LUM

MMP8

SERPINH1

LDHA

D

ADAM12

BMP1

CAPN3

COL18A1

COL5A1

DCN

HSPG2

LAMB1

MMP1

MMP9

SPARC

NONO

E

ADAM15

BMP10

CAPN9

COL1A1

COL5A2

DMD

HTRA1

LAMB2

MMP12

NCSTN

SPP1

PPIH

F

ADAM17

BMP2

CASP3

COL1A2

COL6A1

DMP1

KLK12

LAMB3

MMP13

NID1

TIMP1

GDC

G

ADAM8

BMP4

CAST

COL2A1

COL6A3

FBN1

KLK7

LAMC1

MMP14

NTN4

TIMP2

PPC

H

ADAMTS1

BMP7

CD44

COL3A1

COL7A1

FGA

LAMA1

LAMC2

MMP2

PCOLCE

VCAN

NTC

 

 * gene selection may be updated based on new research and development

 

货号GK105
产地美国
缩写GQH-ECM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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