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人类滋养层细胞生物学qPCR检测试剂盒

英文名:Human Trophoblast Cell Biology qPCR Array Kit

货号:GK102

价格:$250

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Trophoblast cells form the outer layer of the blastocyst and subsequently differentiate into the intermediate trophoblast, cytotrophoblast, and syncytiotrophoblast layers. Trophoblasts play a central role in embryo implantation and interact with the maternal decidual layer of the placenta. ScienCell's GeneQuery™ Human Trophoblast Cell Biology qPCR Array Kit (GQH-TRO) is designed to facilitate gene expression profiling of 88 key genes involved in human trophoblast development, proliferation and differentiation, as well as important pathways in trophoblast cell biology, including LIF-STAT, FGF and NODAL/TGFB signaling pathways. Genes implicated in gestational trophoblastic disease and choriocarcinoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

  • Trophoblast cell markers: KRT7, GATA3, TFAP2C, ELF5, HLA-G, CGA, CGB3, CDX2, EOMES, ITGA1, VIM, POU5F1
  • Development and proliferation: NANOG, SOX2, ESRRB, ASCL2, FLT1, HAND1, BHLHE40, GCM1, NODAL, PCSK6, FURIN
  • Trophoblast differentiation: TCF4, ASCL2, ID1/2, TFAP2C, HAND1, THBD, EIF3A, NCOA6, HSF1, GCM1, DLX3
  • LIF-STAT signaling: IL6ST, LIFR, STAT1/3, SOCS3, TIMP1, ITGB3, JUNB
  • FGF signaling: FGF4, FGFR2, MAPK1/3, FRS2, PTPN11
  • NODAL/TGFB signaling: NODAL, ACVR2A/B, ACVR1B, SMAD2/3
  • Gestational trophoblastic disease: INHA, LNPEP, GPC3, EGFR, ERBB2/3, PAM, CGB3, KRT18, PRPTJ, ALPP
  • Choriocarcinoma: HOPX, HLA-G, GH2, CGA, LNPEP, CYP19A1, ERV3-1, CYP11A1, ABCG2

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Trophoblast Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Noteall gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCG2

BHLHE40

CYP19A1

ERV3-1

FURIN

HSF1

ITGB3

MAPK3

PAM

SLC22A4

TCF4

ACTB

B

ACVR1B

CCL2

DLX3

ERVW-1

GATA3

HSP90B1

JUNB

MGAT4A

PAPPA

SMAD2

TEAD3

GAPDH

C

ACVR2A

CD2AP

EGFR

ESRRB

GCM1

ID1

KHDC3L

NANOG

PCSK6

SMAD3

TFAP2A

LDHA

D

ACVR2B

CDKN1C

EIF3A

ETS2

GH2

ID2

KRT18

NCOA6

PLEKHH1

SMARCA4

TFAP2C

NONO

E

AFP

CDX2

ELF5

FGF4

GPC3

IGF2

KRT7

NLRP2

POU5F1

SOCS3

TFPI2

PPIH

F

ALPP

CGA

EOMES

FGFR2

HAND1

IL6ST

LIFR

NLRP5

PTPN11

SOX2

THBD

GDC

G

ALPPL2

CGB3

ERBB2

FLT1

HLA-G

INHA

LNPEP

NODAL

PTPRJ

STAT1

TIMP1

PPC

H

ASCL2

CYP11A1

ERBB3

FRS2

HOPX

ITGA1

MAPK1

NR0B1

SLC19A1

STAT3

VIM

NTC

 

* gene selection may be updated based on new research and development

货号GK102
产地美国
缩写GQH-TRO
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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