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Human Renal Mesangial Cell Biology qPCR Array Kit

英文名:Human Renal Mesangial Cell Biology qPCR Array Kit

货号:GK093

价格:$250

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ScienCell's GeneQuery™ Human Renal Mesangial Cell Biology qPCR Array Kit (GQH-RMB) is designed to facilitate gene expression profiling of 88 key genes involved in essential mesangial cell biology and functions involved in glomerular physiology and pathophysiology. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Renal mesangial cell markers: ACTA1, FN1, PDGFRB, TNS1, ITGA8
  • Mesangial matrix assembly: SERPINB7, Collagen type 4/5, Laminin α/β/γ, SDC2, HSPG2, CSPG5, NID1, ITGA8
  • Growth factor modulation: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3
  • Vasculogenesis: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF
  • Endocytosis: CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB receptors
  • Transmembrane transporter activity: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2
  • Genes implicated in
    • Mesangial proliferative glomerulonephritis: SERPINB7, IL6, CD79A, CCL2, FGF2, EDN1, CCR5, GAS6, AXL
    • Diffuse mesangial sclerosis: ACTN4, TRPC6, INF2, WT1, PLCE1, PAX2, ARHGDIA, LAMB2, LAMC1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Renal Mesangial Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC9

AP2A1

CCL2

COL5A1

FHL2

IGF1

LAMA2

MFGE8

PDGFRB

SFRP2

TGFBR3

β-actin

B

ACTA1

AP2A2

CCR5

COL5A2

FN1

IL6

LAMA3

MYH11

PIEZO2

SH3KBP1

TNS1

GAPDH

C

ACTN4

AP2B1

CD79A

COL5A3

GAS6

INF2

LAMA4

NGF

PLCE1

SHC3

TRPC1

LDHA

D

ADAM12

AP2M1

CLTA

CSPG5

GDF10

ITGA8

LAMA5

NID1

PLN

SMAD1

TRPC4

NONO

E

ADAMTS1

AP2S1

CLTB

CTGF

GJA5

KCNMB1

LAMB1

NID2

PSEN2

SMAD2

TRPC6

PPIH

F

AFAP1L2

ARHGDIA

CLTC

EDN1

HDAC9

KCNQ5

LAMB2

NRP2

PTN

SMAD3

VCAM1

GDC

G

AGTR1

AXL

COL4A1

ETS1

HOPX

KCNRG

LAMC1

P2RX1

SDC2

TGFBR1

WT1

PPC

H

ANGPT2

CACNA2D1

COL4A2

FGF2

HSPG2

LAMA1

MECOM

PAX2

SERPINB7

TGFBR2

ZFYVE9

NTC

* gene selection may be updated based on new research and development

货号GK093
产地美国
缩写GQH-RMB
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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