我的购物车>> 中国区首家总代 专业十六载 打造细胞生物学精品

您当前所在位置:首 页 > 新威尼斯人 > 基因表达分析 > qPCR检测试剂盒 人胰腺星状细胞生物学qPCR检测试剂盒

人胰腺星状细胞生物学qPCR检测试剂盒

英文名:Human Pancreatic Stellate Cell Biology qPCR Array Kit

货号:GK092

价格:$250

产品说明书下载:

产品预订,提交订单信息之后,我们会第一时间与您取得联系!

ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Pancreatic stellate cell markers: ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF
  • Pancreatic stellate cell activation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2
  • Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s
  • ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
  • Cytokine production: IL1A, IL1B, IL6, PDGFs, TGFBs, TNF
  • p53 signaling pathway: CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3
  • Pancreatic fibrosis and cancer development: ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CDK1

COL2A1

DSP

HIF1A

LAMA2

MMP13

PALLD

RBP1

SPARC

THBS2

β-actin

B

ACTG2

CDK6

COL4A2

EGFR

ICAM1

LAMA3

MMP2

PDGFA

RBP2

SPP1

TIMP1

GAPDH

C

ACVR1B

CDKN1A

CRABP1

EI24

IL1A

LAMB1

MYH11

PDGFB

RBP4

STK11

TNC

LDHA

D

CCKBR

CDKN2A

CRABP2

FOXF1

IL1B

LAMB3

MYH9

PDGFC

RBP5

SYP

TNF

NONO

E

CCNB1

CELSR3

CTGF

GADD45G

IL6

LAMC1

NES

PDGFD

RELN

TGFB1

VCAN

PPIH

F

CCNE1

CHEK1

CYP26A1

GFAP

ITGA7

LHX2

NFKB1

POSTN

RIMS1

TGFB2

VIM

GDC

G

CDH1

CLEC3B

CYP26B1

GNL3

KLF6

LOX

NFKB2

RARA

RRM2

TGFB3

VTN

PPC

H

CDH2

COL11A1

DES

HAS1

LAMA1

LRAT

NGFR

RBBP8

SMAD4

TGFBI

ZMAT3

NTC

* gene selection may be updated based on new research and development

货号GK092
产地美国
缩写GQH-PSB
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
相关产品
货号
产品名称
规格
价格
指令
Baidu
搜狗