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人肝星状细胞生物学qPCR检测试剂盒

英文名:Human Hepatic Stellate Cell Biology qPCR Array Kit

货号:GK091

价格:$250

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ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
  • Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
  • Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
  • Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
  • ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
  • Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
  • Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

COL2A1

CXCL2

FGF1

IGF1

LAMB1

MYH10

NGFR

RARA

SPARC

TIMP1

β-actin

B

B9D1

COL4A2

CXCL3

FGF2

IGF2

LAMB3

MYH11

PDGFA

RBP1

SPP1

TMEM67

GAPDH

C

B9D2

CRABP1

CXCL8

FOXF1

IL1A

LAMC1

MYH9

PDGFB

RBP2

STX2

TNC

LDHA

D

BDNF

CRABP2

CYP26A1

GFAP

ITGB3

LEP

NCAM1

PDGFC

RBP4

SYNM

TNF

NONO

E

CC2D2A

CSF1

CYP26B1

HAS1

KLF6

LHX2

NCAM2

PDGFD

RBP5

SYP

VCAM1

PPIH

F

CCL2

CTGF

DES

HGF

LAMA1

LRAT

NES

PKD1

RELN

TGFB1

VCAN

GDC

G

CDH1

CXCL10

EGF

ICAM1

LAMA2

MMP13

NFKB1

PKHD1

RPGRIP1L

TGFBI

VIM

PPC

H

CLEC3B

CXCL12

F2

IFNG

LAMA3

MMP2

NFKB2

PTN

SLC17A5

THBS2

VTN

NTC

 

* gene selection may be updated based on new research and development

货号GK091
产地美国
缩写GQH-HSB
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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