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人VEGF信号通路qPCR检测试剂盒

英文名:Human VEGF Signaling Pathway qPCR Array Kit

货号:GK088

价格:$250

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ScienCell's GeneQuery™ Human Signaling Pathway qPCR Array Kit (GQH-VEG) profiles 88 key genes involved in VEGF and VEGF-related signaling.  VEGF is a growth factor known for stimulating angiogenesis and vasculogenesis.  As such, it is integral to proper blood vasculature formation during development, maintenance, and wound healing.  Aberrant VEGF signaling is associated with ischemia, diabetic retinopathy, macular degeneration, and solid tumor progression.  Brief examples of how included genes may be grouped according to their function are shown below:

  • Signaling receptors: FLT1, FLT4, KDR, KIT, FGFR1
  • Growth factor ligands: VEGFA, VEGFB, VEGFC, FGF2, PGF
  • Cytoskeletal response: ACTA1, ACTA2, ACTB, ACTG1, ACTG2
  • Adhesion regulation: ITGAV, ITGB1, ITGB3, ITGB5, PTK2
  • Downstream transcription factors: ATF2, FOS, JUN, KRAS, NRAS

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human VEGF Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

ATF2

FGF2

FN1

ITGB3

L1CAM

MAPK3

NRP2

PRKCA

PRKCZ

SOS1

β-actin

B

ACTA2

BAD

FGFR1

FOS

ITGB5

MAP2K1

MAPK8

PGF

PRKCB

PTEN

SOS2

GAPDH

C

ACTB

BCAR1

FGFR2

GNA12

ITPR1

MAP2K2

MAPK9

PIK3C2A

PRKCD

PTK2

SRF

LDHA

D

ACTG1

CASP9

FGFR3

GNA13

ITPR2

MAP2K7

MEF2A

PIK3R2

PRKCE

PXN

TIMP3

NONO

E

ACTG2

CRK

FGFR4

GRB2

JUN

MAPK1

MYL9

PIK3R4

PRKCG

RAF1

VEGFA

PPIH

F

AKT1

CSK

FGFRL1

HRAS

KDR

MAPK10

NOS3

PLA2G4A

PRKCH

ROCK1

VEGFB

GDC

G

AKT2

EGF

FLT1

ITGAV

KIT

MAPK12

NRAS

PLCG1

PRKCI

RRAS

VEGFC

PPC

H

AKT3

ELK1

FLT4

ITGB1

KRAS

MAPK13

NRP1

PLCG2

PRKCQ

SHC1

VEGFD

NTC

 

 * gene selection may be updated based on new research and development

货号GK088
产地美国
缩写GQH-VEG
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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