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人类肥胖症qPCR检测试剂盒

英文名:Human Obesity qPCR Array Kit

货号:GK085

价格:$250

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ScienCell's GeneQuery™ Human Obesity qPCR Array Kit (GQH-OBS) profiles 88 key genes involved in the development of human obesity.  Obesity occurs when excess body fat accumulates to the extent that it impacts health negatively.  It is generally determined by measuring body mass index and is most commonly caused by environmental and lifestyle factors although genetic components can confer susceptibility.  Effects of obesity include the increased likelihood of acquiring heart diseases, diabetes, sleeping disorders, cartilaginous diseases, and certain types of cancer.  Brief examples of how included genes may be grouped according to their role in establishing obesity shown below:

  • Markers and susceptibility: ADRB2, ENPP1, GHRL, MC4R, POMC, UCP1
  • Adipogenesis: CREB1, LEP, LPL, PLIN2, PPARG
  • Lipid and cholesterol metabolism: ADIPOQ, CETP, CYP19A1, PPARGC1B
  • Lipid transport: APOL2, APOL3, APOL6, CRABP2, NPC2
  • Intracellular lipid and fatty acid metabolism: ACADL, ALOX5, LRP1, MAL, SCD

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Obesity qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCA1

ANGPTL4

APOL4

CRABP2

ECH1

GCG

HSD11B1

MAL

NR1H3

PPARGC1A

SORL1

β-actin

B

ACADL

APOA5

APOL6

CREB1

ENPP1

GDF10

IGF1

MC4R

NTRK2

PPARGC1B

SREBF1

GAPDH

C

ACADM

APOB

BMP1

CYP19A1

EPAS1

GHRL

INS

MEF2A

PLIN2

RETN

TWIST1

LDHA

D

ADIPOQ

APOC2

CETP

CYP4A11

FASN

GK

LEP

MTTP

PLTP

SCD

UCP1

NONO

E

ADIPOR2

APOE

CIDEA

CYP7A1

FGFR4

GNB3

LEPR

NCOR2

POMC

SERPINE1

UCP2

PPIH

F

ADRB2

APOL1

CLOCK

DDIT3

FOXO1

GPAM

LIPC

NPC2

PON1

SLC27A1

UCP3

GDC

G

ADRB3

APOL2

CNR1

DHCR24

FZD1

GPD1

LPL

NPY

PPARD

SLC27A4

VLDLR

PPC

H

ALOX5

APOL3

CPT2

DLK1

GATA2

HMGCR

LRP1

NR1D2

PPARG

SLC2A4

XBP1

NTC

 

 * gene selection may be updated based on new research and development

货号GK085
产地美国
缩写GQH-OBS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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