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人类周细胞生物学qPCR检测试剂盒

英文名:Human Pericyte Biology qPCR Array Kit

货号:GK081

价格:$250

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ScienCell's GeneQuery™ Human Pericyte Biology qPCR Array Kit (GQH-PER) profiles 88 key genes involved in pericyte function during angiogenesis and in immunity.  In vasculature, pericytes function as mural cells on small blood vessels and they help maintain the blood-brain barrier (BBB), an integral homeostatic brain function.  Pericytes also possess macrophage-like properties when functioning in the immune response.  Defects in pericyte function can result in BBB breakdown and aberrant vasculature formation.  Below are brief examples of how included genes may be grouped according to their function in pericyte biology:

  • Angiogenesis: VEGFA, HIF1A, JAG1, CTGF, ANGPT2
  • BBB maintenance: ABCC9, VCAM1, OCLN, ICAM1, DLK1
  • Inflammation: RIPK2, TLR3, CD74, CXCL9, APOL3
  • Scavenging: FCGR2A, HLA-E, ITGAM, C3, CD36
  • Markers: CSPG4 (NG2), PDGFRB, MCAM, RGS5, CD248

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Pericyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC9

APOL3

CD4

CSPG4

CXCL9

EGFR

HIF1A

ITGAM

NOTCH1

PTGS2

TLR3

β-actin

B

ACTA2

ARNT2

CD40

CTGF

CXCR3

EPHA2

HLA-DMA

JAG1

NOTCH3

RET

TNC

GAPDH

C

ADM

C1R

CD47

CXCL1

DES

ERBB2

HLA-E

KCNJ8

NOX4

RGS5

TNFRSF10D

LDHA

D

AGTR1

C3

CD68

CXCL10

DLK1

FCAR

ICAM1

MCAM

OCLN

RIPK2

TNFRSF14

NONO

E

ANGPT1

CCL2

CD74

CXCL11

EDN1

FCGR1A

IL32

MMP2

PDGFRB

SOD2

TPM1

PPIH

F

ANGPT2

CCL8

CDH2

CXCL12

EDNRA

FCGR2A

IL6

MYLK

PECAM1

TGFB1

VCAM1

GDC

G

ANPEP

CD248

CREB5

CXCL2

EDNRB

GJB6

IRF1

NFKB1

PRKAA1

TGFB3

VEGFA

PPC

H

APOL2

CD36

CREBBP

CXCL8

EFNB2

HBEGF

ITGA5

NOS2

PSMB8

TGFBR1

VEGFC

NTC 


 * gene selection may be updated based on new research and development

货号GK081
产地美国
缩写GQH-PER
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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