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人角质细胞生物学qPCR检测

英文名:Human Keratocyte Biology qPCR Array

货号:GK074

价格:$250

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ScienCell's GeneQuery™ Human Keratocyte Biology qPCR Array (GQH-KER) profiles 88 key genes involved in corneal keratocyte function.  Keratocytes are specialized fibroblasts that play roles in wound healing, maintaining corneal transparency, and corneal component synthesis.  Keratocyte pathologies include keratoconus and limbal stem cell deficiency.  Below are brief examples of how included genes may be grouped according to their function in keratocyte biology:

  • Markers: MUC1, CD34, KERA, KRT19, LUM
  • Wound Healing: MMP12, MRC1, NFKB1, COL3A1, CCL2
  • Cell Cycling: MAPK1, MYCN, PTN, ETS1, CAPN9
  • Oxidative Stress Response: RRAS, NOS2, NQO1, MAF, HMOX1
  • Keratoconus Development: IGFBP3, MMP1, SSTR1, CRX, TNFAIP6

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Keratocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CCL2

COL1A1

CRYAA

F13A1

GSTK1

KERA

MAPK3

NCAM1

PTN

TGFBR1

β-actin

B

ACTA2

CCL7

COL1A2

CRYAB

FASLG

HMOX1

KRT1

MMP1

NFKB1

RRAS

TGFBR2

GAPDH

C

ALDH1A1

CD34

COL3A1

CRYBB1

FGF2

ICAM1

KRT12

MMP12

NFKB2

SDC1

TIMP1

LDHA

D

ATF1

CD68

COL5A1

CRYGD

FGFR1

IGF1R

KRT19

MMP2

NOS2

SOD2

TIMP2

NONO

E

BGN

CDK1

COL5A2

CXADR

FGFR2

IGFBP3

LCN2

MMP3

NQO1

SPARC

TIMP3

PPIH

F

BMP4

CFL1

COL6A2

ECM1

FN1

IGFBP5

LUM

MRC1

PCNA

SSTR1

TKT

GDC

G

C12orf57

CHST6

COL7A1

EPHX1

FOS

IL1A

MAF

MUC1

PDGFRB

TC2N

TNFAIP6

PPC

H

CAPN9

CLU

CRX

ETS1

FOSL1

IL6

MAPK1

MYCN

PTGDS

TGFB1

VCL

NTC

  * gene selection may be updated based on new research and development

货号GK074
产地美国
缩写GQH-KER
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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