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人类软骨细胞通道和受体qPCR检测试剂盒

英文名:Human Chondrocyte Channels and Receptors qPCR Array Kit

货号:GK068

价格:$250

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The primary function of chondrocytes is to synthesize and secrete cartilage matrix during injury and repair.  To perform this task, chondrocytes need to sense changes in their surrounding cartilage ECM, therefore chondrocyte mechanotransduction is a key component of healthy articular cartilage function.  ScienCell's GeneQuery™ Human Chondrocyte Channels and Receptors qPCR Array (GQH-CCR) facilitates gene expression profiling of 88 channels and receptors involved in chondrocyte mechanotransduction and environment sensing.  Brief examples of how included genes may be grouped according to channel or receptor type are shown below:

  • Potassium Channels: KCNA4, KCNJ8, KCNK5, ABCC9, ABCC8
  • Chloride Channels: ANO1, ANO3, ANO5, CFTR, CLCN3
  • G protein-coupled receptors: HRH1, PTGER1, ADORA2A, P2RX1, P2RY1
  • NMDA receptors: GRIN1, GRIN2A, GRIN2B, GRIN2D
  • Chemokine receptors: CCR1, CCR2, CCR5, CXCR1, CXCR2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Chondrocyte Channels and Receptors qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC8

ASIC1

CCR1

CXCR1

GRIN2B

ITGA2

ITGB5

LEPR

P2RY1

PTGER4

TLR3

β-actin

B

ABCC9

CACNA1A

CCR2

CXCR2

GRIN2D

ITGA3

KCNA4

MC1R

P2RY2

PTH1R

TLR4

GAPDH

C

ADORA2A

CACNA1C

CCR3

ESR1

HRH1

ITGA4

KCNA6

MC2R

PIEZO1

SCN9A

TLR9

LDHA

D

ANO1

CACNA1D

CCR5

ESR2

HRH2

ITGA5

KCNJ11

MC5R

PIEZO2

SCNN1A

TRPV1

NONO

E

ANO3

CACNA1E

CD36

FGFR2

HRH4

ITGA6

KCNJ8

P2RX1

PPARG

SCNN1B

TRPV2

PPIH

F

ANO5

CACNA1G

CD44

FGFR3

IGF1R

ITGA7

KCNK5

P2RX3

PTGER1

SCNN1D

TRPV3

GDC

G

ANO6

CACNA1H

CFTR

GRIN1

INSR

ITGAV

KCNMB1

P2RX4

PTGER2

SCNN1G

TRPV4

PPC

H

AQP1

CACNA1I

CLCN3

GRIN2A

ITGA1

ITGB1

KCNN4

P2RX7

PTGER3

TGFBR2

TRPV6

NTC

 * gene selection may be updated based on new research and development

货号GK068
产地美国
缩写GQH-CCR
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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