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人类细胞生长和分裂qPCR检测试剂盒

英文名:Human Cell Growth and Division qPCR Array Kit

货号:GK062

价格:$250

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ScienCell's GeneQuery™ Human Cell Growth and Division qPCR Array Kit (GQH-CGD) is designed to facilitate gene expression profiling of 88 key genes involved in cell growth and division. Tight control of these processes is essential to ensure that the hereditary information is correctly passed on from parent cells to daughter cells, and that cell growth is strictly regulated. Failure of such control gives rise to mutations and/or tumorigenesis. Brief examples of how included genes may be grouped are shown below:     

  • Cell cycle regulation: ATM, CCNA2, CCNB1, CCNE2, CDC20, CDC25C, CDK1, CDK2, CHEK1, NBN, RAD1, RAD17
  • Mitosis: APC/C complex, CDC genes, BUB1, BUB1B, EGF, EREG, KNTC1, NPM2, MAD2L1, MAD2L2, PKMYT1, TGFA, TGFB1, TTK
  • Meiosis: APC/C complex, CDC genes, RAD51, RBX1, SKP1, SMC1A, SMC3
  • Spindle checkpoint: AURKA, BIRC5, BUB1, CDC16, CDC27, DCTN1, KNTC1, PAFAH1B1, SMC1A, SMC3, STMN1, TTK

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Cell Growth and Division qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY1

ANAPC2

BUB1

CCNE2

CDC27

EGF

KNTC1

NBN

PPP3CA

RAD17

TARDBP

β-actin

B

ADCY2

ANAPC4

BUB1B

CD28

CDK1

EREG

MAD2L1

NPM2

PPP3R1

RAD51

TGFA

GAPDH

C

ADCY3

ANAPC5

CALM1

CDC16

CDK2

ESPL1

MAD2L2

PAFAH1B1

PRKACA

RBX1

TGFB1

LDHA

D

ADCY8

ANAPC7

CAMK2A

CDC20

CDKN2B

FBXW11

MAP2K1

PBRM1

PRKACB

RPS6KA1

TTK

NONO

E

ANAPC1

ATM

CAMK2G

CDC23

CHEK1

IGF1

MAPK1

PKMYT1

PRKACG

SKP1

TTN

PPIH

F

ANAPC10

AURKA

CCNA2

CDC25B

CHP2

IGF1R

MAPK12

PLK1

PRKX

SMC1A

YWHAB

GDC

G

ANAPC11

BIRC5

CCNB1

CDC25C

CUL1

ITPR1

MAPK3

PPP1CA

PTTG1

SMC3

YWHAE

PPC

H

ANAPC13

BTRC

CCNB2

CDC26

DCTN1

ITPR2

MRE11A

PPP2CA

RAD1

STMN1

YWHAG

NTC

 

* gene selection may be updated based on new research and development

货号GK062
产地美国
缩写GQH-CDG
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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