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人类DNA损伤感应qPCR检测试剂盒

英文名:Human DNA Damage Sensing qPCR Array Kit

货号:GK061

价格:$250

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ScienCell's GeneQuery™ Human DNA Damage Sensing qPCR Array Kit (GQH-DDS) is designed to facilitate gene expression profiling of 40 key genes involved in DNA damage sensing. DNA damages, such as mutations, breaks, cross-links, deletions/insertions and translocations, are constantly generated due to internal and external stresses. Appropriate DNA damage responses must be implemented to maintain genome stability. Brief examples of how included genes may be grouped are shown below:

  • DNA damage sensors: ATM, ATR, PRKDC, PARP1
  • Cell cycle regulation: CHEK1, CHEK2, CCNB1, CCNB2, CCNB3, CCND1, CCND2, CCND3, CCNE2, CDK2, CDK4, CDK6
  • ATM signaling pathway: ABL1, BRCA1, CDC25A, CDKN1A, CHEK1, CHEK2, GADD45A, RAD51, RBBP8, TP53
  • ATR signaling pathway: CDC25A, CDC25C, CDK2, CHEK1, CHEK2, HUS1, MDM2, RAD1, RAD17, RAD9A, TP53
  • Apoptosis: ABL1, ATM, BAX, BRCA1, CASP3, CASP9, CDKN1A, CHEK2, PRKDC, RAD91, TP53

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human DNA Damage Sensing qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABL1

CCNB1

CDC25C

FAS

PRKDC

β-actin

ABL1

CCNB1

CDC25C

FAS

PRKDC

β-actin

B

ATM

CCNB2

CDK2

GADD45A

RAD1

GAPDH

ATM

CCNB2

CDK2

GADD45A

RAD1

GAPDH

C

ATR

CCNB3

CDK4

GADD45B

RAD17

LDHA

ATR

CCNB3

CDK4

GADD45B

RAD17

LDHA

D

BAX

CCND1

CDK6

HUS1

RAD51

NONO

BAX

CCND1

CDK6

HUS1

RAD51

NONO

E

BID

CCND2

CDKN1A

MDM2

RAD9A

PPIH

BID

CCND2

CDKN1A

MDM2

RAD9A

PPIH

F

BRCA1

CCND3

CDKN1B

MYC

RB1

GDC

BRCA1

CCND3

CDKN1B

MYC

RB1

GDC

G

CASP3

CCNE2

CHEK1

NBN

RBBP8

PPC

CASP3

CCNE2

CHEK1

NBN

RBBP8

PPC

H

CASP9

CDC25A

CHEK2

PARP1

TP53

NTC

CASP9

CDC25A

CHEK2

PARP1

TP53

NTC

 

* gene selection may be updated based on new research and development

货号GK061
产地美国
缩写GQH-DDS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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