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人类昼夜节律qPCR检测试剂盒

英文名:Human Circadian Rhythm qPCR Array Kit

货号:GK060

价格:$250

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ScienCell's GeneQuery™ Human Circadian Rhythm qPCR Array Kit (GQH-CIR) profiles 88 key genes involved in the regulating changes that occur on a roughly 24-hr cycle.  Circadian rhythms are primarily responses to changes in light and are found in most living organisms.  Not to be confused with biological clocks, circadian rhythms can influence sleep patterns, hormone release, body temperature, and other homeostatic functions.  Below are brief examples of how included genes may be grouped according to their function:

  • Clock regulation: ARNTL, BHLHE40, CLOCK, PER1, RORA
  • Casein kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1
  • CREB pathway: CAMK2A, HTR7, MAPK1, PRKACB, AANAT
  • Transcription factors: SMAD4, IRF1, NFIL3, RORC, SP1,

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Circadian Rhythm qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AANAT

BHLHE41

CREB3

CSNK2A1

HDAC1

KLF9

MYOD1

NR2F6

PRKAB1

PRKCA

RORC

β-actin

B

ADORA1

BTRC

CREBBP

CUL1

HDAC2

KMT2A

NCOA2

PER1

PRKAB2

PRKCB

SIN3A

GAPDH

C

ADORA2A

CAMK2A

CRX

DBP

HEBP1

LGR4

NFIL3

PER2

PRKACB

PROK2

SKP1

LDHA

D

AHR

CAMK2B

CRY1

EGR1

HTR7

MAPK1

NGFR

PER3

PRKACG

PROX1

SMAD4

NONO

E

ARNTL

CDK5R1

CRY2

EGR3

ID1

MAPK14

NOCT

PPARA

PRKAG2

RBX1

SP1

PPIH

F

ARNTL2

CHEK1

CSNK1A1

EPO

ID2

MAPK3

NONO

PPARGC1A

PRKAG3

ROCK2

SREBF1

GDC

G

ATR

CLOCK

CSNK1D

FBXL3

IRF1

MMAB

NPAS2

PRKAA1

PRKAR1A

RORA

STAT5A

PPC

H

BHLHE40

CREB1

CSNK1E

FBXW11

KLF10

MTNR1B

NR1D2

PRKAA2

PRKAR2A

RORB

UBE3A

NTC 


 * gene selection may be updated based on new research and development

货号GK060
产地美国
缩写GQH-CIR
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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