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糖尿病qPCR检测试剂盒的人类并发症

英文名:Human Complications of Diabetes qPCR Array Kit

货号:GK052

价格:$250

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ScienCell's GeneQuery™ Human Complications of Diabetes qPCR Array (GQH-DBC) contains primers for 88 genes commonly misregulated in diabetic complications.  Diabetes increases the risk of developing a number of long-term complications that arise gradually.  Such complications include but are not limited to retinopathy, angiopathy, nephropathy, macular edema, ketoacidosis, and neuropathy.  Brief examples of how included genes may be grouped according to complication are shown below:

  • Retinopathy: AKR1B1, ANGPT2, HFE, NAGPA, VEGFA
  • Neuropathy: SOD2, SYNM, TRPV1, GDNF, DBH, ACE
  • Macular edema: CCL2, EPO, ICAM1, IL6, REN
  • Nephropathy: TGFB1, NAGLU, IL1RN, CTGF, AGT
  • Ischemic stroke: MTHFR, PLAT, PTGIS, F2, APOH

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Complications of Diabetes qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACE

ANGPT2

CNTF

FGF2

GDNF

IGF1R

LEP

NOS3

PLG

SELE

TGFB1

β-actin

B

ADIPOQ

AOC3

CTGF

FGFR2

HBA2

IGFBP7

LMNA

NOTCH3

PLXDC2

SERPINF1

THBD

GAPDH

C

AGER

APOH

DBH

FGFR3

HFE

IL10

MPZ

NTF3

PMP22

SOD2

TNFRSF11B

LDHA

D

AGT

CALCA

EDN1

FLT1

HIF1A

IL1RN

MTHFR

NTRK1

PRKCB

SOD3

TPO

NONO

E

AGTR1

CAT

EPCAM

FN1

HLA-DQB1

IL6

NAGLU

PARP1

PRKCH

SST

TRPV1

PPIH

F

AKR1B1

CCL2

EPO

G6PD

HLCS

INS

NAGPA

PDGFRB

PTGIS

SYNM

VCAM1

GDC

G

ALB

CDH5

F2

GAD1

ICAM1

INSR

NGF

PGF

REN

TEK

VEGFA

PPC

H

ALOX5AP

CHN2

F5

GAD2

IGF1

KDR

NGFR

PLAT

RHO

TG

VWF

NTC 

 * gene selection may be updated based on new research and development

货号GK052
产地美国
缩写GQH-DBC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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