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人类糖尿病,II型(迟发性,胰岛素抵抗)qPCR检测试剂盒

英文名:Human Diabetes, Type II (Late-onset, Insulin Resistance) qPCR Array Kit

货号:GK051

价格:$250

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ScienCell's GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin Resistance) qPCR Array Kit (GQH-DB2) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of late-onset diabetes mellitus.  Type 2 diabetes mellitus is characterized by abnormally high blood sugar levels resulting from a failure to utilize insulin properly.  Brief examples of how included genes may be grouped according to function are shown below:

  • Blood glucose regulation: INS, INSR, SLC2A2, SLC30A8, ABCC8
  • Fat regulation: ADIPOQ, LPL, PON1, LEP, RETN
  • Cholesterol regulation: APOA1, APOB, LPA, CETP
  • Metabolism: UCP2, GCG, PCK2, GYS1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin resistance) qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC8

AKT2

CETP

GCK

HNF1A

IGFBP3

LEP

NEUROD1

PON1

SELE

TCF4

β-actin

B

ACE

ALB

COG2

GFPT1

HNF1B

IL6

LEPR

NOS3

PPARA

SERPINA12

TCF7L2

GAPDH

C

ADIPOQ

ALKBH3

CRP

GHRL

HNF4A

IMMP2L

LIPC

PAX4

PPARG

SERPINE1

TNF

LDHA

D

ADIPOR1

APOA1

DPP4

GPD2

HSD11B1

INS

LIPH

PCK2

PPARGC1A

SLC2A1

UCP2

NONO

E

ADRB3

APOB

EDN1

GYS1

IAPP

INSR

LPA

PDX1

PPP1R3A

SLC2A2

UCP3

PPIH

F

AGT

APOC3

ENPP1

HES1

IGF1

IRS1

LPL

PIK3R1

PTPN1

SLC2A4

VCAM1

GDC

G

AGTR1

CAPN10

GAD2

HK2

IGF2

IRS2

MAPK8IP1

PLAT

RBP4

SLC30A8

VWF

PPC

H

AKT1

CCL2

GCG

HMGA1

IGFBP1

KCNJ11

MTNR1B

PLTP

RETN

SOCS3

WFS1

NTC

* gene selection may be updated based on new research and development

货号GK051
产地美国
缩写GQH-DB2
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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