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人体皮肤伤口愈合qPCR检测套件

英文名:Human Skin Wound Healing qPCR Array Kit

货号:GK041

价格:$250

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ScienCell's GeneQuery™ Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Wound healing stages
    • Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
    • Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
    • Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
  • Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
  • Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
  • Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
  • Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Skin Wound Healing qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

COL1A2

CSK

CXCL8

FGF2

IL10

ITGB1

MMP7

PDGFRB

RAC1

TGFBR3

β-actin

B

ACTC1

COL3A1

CTGF

EGF

FGF7

IL1A

ITGB3

MMP9

PLAT

RHOA

TIMP1

GAPDH

C

ANGPT1

COL4A1

CTNNB1

EGFR

FIGF

IL1B

ITGB5

NGF

PLAU

SERPINE1

TNF

LDHA

D

CCL2

COL4A3

CTSG

ELANE

HBEGF

IL4

ITGB6

PDGFA

PLAUR

STAT3

VEGFA

NONO

E

CD40LG

COL5A1

CTSK

F13A1

HGF

IL6

MAPK1

PDGFB

PLG

TAGLN

VEGFB

PPIH

F

CDC42

COL5A3

CTSV

F3

HIF1A

IL6ST

MAPK3

PDGFC

PRTN3

TGFA

VEGFC

GDC

G

CDH1

CSF2

CXCL1

FGA

IFNG

ITGA1

MMP1

PDGFD

PTEN

TGFB1

WISP1

PPC

H

COL14A1

CSF3

CXCL2

FGF10

IGF1

ITGA5

MMP2

PDGFRA

PTGS2

TGFB2

WNT5A

NTC

 * gene selection may be updated based on new research and development

货号GK041
产地美国
缩写GQH-SWH
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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