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人类实体肿瘤中转录失调qPCR检测试剂盒

英文名:Human Transcriptional Misregulation in Solid Tumors qPCR Array Kit

货号:GK031

价格:$250

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ScienCell's GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Kit (GQH-TMS) is designed to facilitate gene expression profiling of 88 key genes involved in six types of solid tumor development (prostate cancer, kidney cancer, thyroid cancer, neuroblastoma, carcinoid, and sarcomas). Brief examples of how included genes may be grouped according to function and tumor types are shown below:     

  • Cell cycle regulation: CDK9, CDKN1A, CDKN1B
  • Apoptosis and proliferation: TP53, RXRs, PPARG, MDM2
  • Cellular invasion: MMP9, SPINT1, IL1R2, PLAU, PLAT, ZEB1
  • Cell survival: BMI1, MYC, IGF1, MLF1
  • Mutated transcription factors identified in
    • prostate cancer: ERG, ETV1, ETV4, ELK4
    • kidney cancer: TFE3
    • thyroid cancer: PAX8
    • neuroblastoma: MYCN
    • sarcomas: ATF1, FEV, WTI, DDIT3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AFF1

CCNT2

DDX5

FUS

HIST1H3D

HIST2H3D

MAX

MYC

PAX8

SLC45A3

TFE3

β-actin

B

ARNT2

CDK9

ELK4

GOLPH3

HIST1H3E

HIST3H3

MDM2

MYCN

PLAT

SMAD1

TGFBR2

GAPDH

C

ASPSCR1

CDKN1A

ERG

GOLPH3L

HIST1H3F

IGF1

MEN1

NFKB1

PLAU

SP1

TMPRSS2

LDHA

D

ATF1

CDKN1B

ETV1

H3F3B

HIST1H3G

IGF1R

MET

NFKBIZ

PPARG

SPINT1

TP53

NONO

E

ATM

CEBPB

ETV4

H3F3C

HIST1H3H

IL1R2

MLF1

NGFR

PRCC

SS18

TSPAN7

PPIH

F

BAIAP3

COMMD3

EWSR1

HIST1H3A

HIST1H3I

IL2RB

MLLT1

NTRK1

RXRA

SSX2

WT1

GDC

G

BMI1

CXCL8

FEV

HIST1H3B

HIST1H3J

IL6

MLLT3

NUPR1

RXRB

SSX2B

ZBTB17

PPC

H

CCNT1

DDIT3

FOXO1

HIST1H3C

HIST2H3A

KMT2A

MMP9

PAX7

RXRG

TAF15

ZEB1

NTC

 

* gene selection may be updated based on new research and development

货号GK031
产地美国
缩写GQH-TMS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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