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人类非经典Wnt信号通路qPCR检测试剂盒

英文名:Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

货号:GK027

价格:$250

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ScienCell's GeneQuery™ Human Noncanonical Wnt Signaling Pathways qPCR Array Kit (GQH-WAP) is designed to facilitate gene expression profiling of 88 key genes involved in alternative Wnt pathways.  This kit emphasizes genes involved in planar cell polarity signaling and calcium signal transduction.  Brief examples of how included genes may be grouped according to function are shown below:

  • Planar cell polarity: DAAM1, CELSR1, FAT1, FZD7, VANGL1
  • Calcium: CASR, CALM1, FOSL1, FZD4, NFATC1
  • Signaling modulator: LRP5, LRP6, SPRY1, EFNB, CASR
  • Receptors: WNT2B, WNT4, WNT5A, WNT9A, WNT11
  • Target genes: RHOA, RACGAP1, CREBBP, CFL1, FUZ

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Noncanonical Wnt Signaling Pathways qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ATN1

CAPN2

CREBBP

DGKE

FAT4

FZD6

ITPR3

PFN1

RACGAP1

SPRY1

WNT4

β-actin

B

AXIN1

CASR

CREBZF

DVL1

FLNA

FZD7

LIMK1

PLCD1

RGS18

TLR4

WNT5A

GAPDH

C

AXIN2

CDX2

CSNK1E

DVL2

FOSL1

GSK3B

LRP5

PLCE1

RGS3

VANGL1

WNT5B

LDHA

D

CALM1

CELSR1

DAAM1

DVL3

FUZ

HGS

LRP6

PLCG1

RHOA

VANGL2

WNT6

NONO

E

CAMK2A

CELSR2

DCHS1

EFNB1

FZD2

INTU

MAPK8

PRICKLE1

ROR1

WNT11

WNT7A

PPIH

F

CAMK2B

CELSR3

DCHS2

FAT1

FZD3

INVS

NFATC1

PRICKLE2

ROR2

WNT16

WNT7B

GDC

G

CAMK4

CFL1

DGKB

FAT2

FZD4

ITPR1

NFATC2

PRKCD

RYK

WNT2B

WNT9A

PPC

H

CAPN1

CFL2

DGKD

FAT3

FZD5

ITPR2

PCDH8

PTK7

SIAH2

WNT3A

WNT9B

NTC

 

 * gene selection may be updated based on new research and development

货号GK027
产地美国
缩写GQH-WAP
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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