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人内皮细胞生物学qPCR检测试剂盒

英文名:Human Endothelial Cell Biology qPCR Array Kit

货号:GK019

价格:$250

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ScienCell's GeneQuery™ Human Endothelial Cell Biology qPCR Array Kit (GQH-END) is designed to facilitate gene expression profiling of 88 key genes to help characterize endothelial cells.  Examining the expression levels of included genes can help determine endothelial cell status and condition.  Brief examples of how included genes may be grouped according to function are shown below:

  • Junctional proteins: CDH2, CDH5, CLDN5, GJA1, VCL
  • Motility: BMP1, MMP2, ITGB1, ITGB3, SEMA3F
  • Cell cycling, growth, and death: CDC42, FOXO1, ALK1, SOD1, CFLAR
  • Inflammatory response: STAT1, EDNRA, PDGFB, ACE, TRADD
  • Coagulation response: SERPINE1, PLAT, SELP, NOD1, CD40

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Endothelial Cell Biology qPCR Array Plate Layout* (8 controls in bold and italic): 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACE

BMP1

CFLAR

EFNB2

GJA1

ITGB5

MMP2

PARD3

PROCR

STAT1

TNFRSF1A

ACTB

B

ADAM15

CASP3

CLDN5

ENG

GJA5

JUN

NFKB1

PARD6A

PTPRJ

STAT3

TNFRSF1B

GAPDH

C

ADAM17

CD34

CRB1

EREG

HIF1A

KDR

NOD1

PDGFA

RHOA

STAT4

TRADD

LDHA

D

ADAMTS1

CD40

CTNNB1

F11R

HOXD10

LGALS9

NOD2

PDGFB

S1PR1

THBS1

VCAM1

NONO

E

SLPI

CD82

CTNND1

F2R

ID1

MADCAM1

NOS2

PECAM1

SELP

TIMP1

VCL

PPIH

F

ALOX5

CDC42

CX3CL1

FLT1

ILK

MAPK8

NOS3

PIK3CA

SEMA3F

TJP1

VWF

GDC

G

APOE

CDH2

DLL4

FLT4

ITGB1

MCAM

NOTCH1

PLAT

SERPINE1

TLR2

WNK4

PPC

H

BCL2

CDH5

EDNRA

FOXO1

ITGB3

MMP1

OCLN

PODXL

SOD1

TLR4

YAP1

NTC

 

* gene selection may be updated based on new research and development

货号GK019
产地美国
缩写GQH-END
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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