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人内皮细胞表型异质性qPCR检测试剂盒

英文名:Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

货号:GK018

价格:$250

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ScienCell's GeneQuery™ Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit (GQH-EPH) is designed to facilitate gene expression profiling of 88 key genes that are differentially expressed in vascular endothelial cells, and in endothelial cells from various tissues. Brief examples of how included genes may be grouped according to vascular types and tissue origins are shown below:     

  • Endothelial cells vs. non-endothelial cells: PECAM1, CDH5, MMRN1, EFEMP1, VWF
  • Artery cells vs. vein cells: ALDH1A1, MPZL2, LIPG, ITM2A, CD44, MYO1B, GDF1, LEFTY1, SMO, EPHB4
  • Large vessel cells vs. microvessel cells: SPARC, JAM3, VEGFC, COL5A1, ROBO1, MYLK, COL4A3BP, EDNRB, VAV3, PDGFD
  • Tissue origin:
    • skin: FGF2, SCD, HMGCS1
    • lung: SFRP1, OGN
    • uterus: GAL
    • intestine: BTD

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABLIM1

CD36

COL5A2

ESR2

GFRA2

ITGB4

LIPG

MYO1B

OGN

PRKACA

SPARC

β-actin

B

ABLIM2

CD44

CTGF

FABP4

GMFG

ITM2A

MCAM

MYO5C

PAK2

PROCR

SSH1

GAPDH

C

ABLIM3

CDH5

EDN1

FABP5

HEY2

JAM3

MGP

MYO7A

PALMD

ROBO1

TGFA

LDHA

D

ALDH1A1

CEACAM1

EDNRB

FABP7

HHIP

KDR

MMRN1

NAV1

PDGFD

SCD

THBS1

NONO

E

ANGPT2

COL4A1

EFEMP1

FGF2

HMGCS1

KRT7

MPZL2

NAV3

PECAM1

SEC14L1

TMSB4X

PPIH

F

APOD

COL4A2

EFNA1

GAL

IL1R1

LAMA4

MRC1

NOTCH4

PGF

SFRP1

VAV3

GDC

G

BMP6

COL4A3BP

EPHB4

GDF1

IL6R

LDB2

MRC2

NRG1

PODXL

SMO

VEGFC

PPC

H

BTD

COL5A1

ESM1

GFRA1

ITGA1

LEFTY1

MYLK

NTN4

PPP1R16B

SORBS2

VWF

NTC

* gene selection may be updated based on new research and development

货号GK018
产地美国
缩写GQH-EPH
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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